Restriction Enzymes
New England Biolabs, Inc. T7 Endonuclease I – 250 units
T7 Endonuclease I recognizes and cleaves non-perfectly matched DNA, cruciform DNA structures, Holliday structures or junctions, heteroduplex DNA and more slowly, nicked double-stranded DNA. The cleavage site is at the first, second or third phosphodiester bond that is 5' to the mismatch. The protein is the product of T7 gene 3.
Worthington Biochemical Corporation DEOXYRIBONUCLEASE I FLTRD-25MG
Filtered through a 0.22 micron membrane and lyophilized in vials. Material is not tested for pyrogenicity.
New England Biolabs, Inc. BspQI – 500 units
A restriction endonuclease that recognizes the sequence GCTCTTCN^NNN_.
New England Biolabs, Inc. PvuI-HF – 2500 units
A high fidelity restriction endonuclease that recognizes the sequence CG_AT^CG. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes. Examples of nuclease contamination studies for some of our HF restriction enzymes are shown below.
New England Biolabs, Inc. NmeAIII – 250 units
A restriction endonuclease that recognizes the sequence GCCGAGNNNNNNNNNNNNNNNNNNN_NN^.
New England Biolabs, Inc. CviQI – 10000 units
A restriction endonuclease that recognizes the sequence G^TA_C.
New England Biolabs, Inc. PciI – 1000 units
A restriction endonuclease that recognizes the sequence A^CATG_T.
New England Biolabs, Inc. BsrI – 5000 units
A restriction endonuclease that recognizes the sequence ACTG_GN^.
New England Biolabs, Inc. APE 1 – 5000 units
Human apurinic/apyrimidinic (AP) endonuclease, APE 1, also known as HAP 1 or Ref-1, shares homology with Escherichia coli exonuclease III protein. APE 1 cleaves the phosphodiester backbone immediately 5 to an AP site, via hydrolytic mechanism, to generate a single-strand DNA break leaving a 3-hydroxyl and 5-deoxyribose phosphate terminus. Besides AP endonuclease activity, APE 1 has also been reported to have weak DNA 3 -diesterase, 3 to 5 exonuclease and RNase H activities (3-5). In addition to DNA repair activity, APE 1 is also capable of regulating the DNA binding activity of many transcription factors in vitro by a redox mechanism. As part of this process, APE 1 has been shown to stimulate the DNA binding activity of Fos-Jun heterodimers, Jun-Jun homodimers and Hela cell AP-1 proteins as well as that of several other transcription factors including NF-kB, Myb and members of the ATF/CREB family.
New England Biolabs, Inc. BspEI – 5000 units
A restriction endonuclease that recognizes the sequence T^CCGG_A.
New England Biolabs, Inc. BslI – 5000 units
A restriction endonuclease that recognizes the sequence CCNN_NNN^NNGG.
New England Biolabs, Inc. I-SceI – 500 units
A restriction endonuclease that recognizes the sequence TAGGG_ATAA^CAGGGTAAT. I-SceI is an intron-encoded endonuclease. The intron encoding I-SceI is present in the mitochondria of Saccharomyces cerevisiae.
New England Biolabs, Inc. BpmI – 100 units
A restriction endonuclease that recognizes the sequence CTGGAGNNNNNNNNNNNNNN_NN^.
New England Biolabs, Inc. Acc65I – 10000 units
A restriction endonuclease that recognizes the sequence G^GTAC_C.
New England Biolabs, Inc. EciI – 100 units
A restriction endonuclease that recognizes the sequence GGCGGANNNNNNNNN_NN^.